I got a jacket and on that jacket is what I think is blood, 150ml atleast. How do I know for sure?

Luminol Test
◦How it works: A chemical compound, known as Luminol, is used in solution or sprayed onto suspected surfaces. This compound gives a strong blue fluorescence when viewed with a UV light. The Luminol reacts with hematin, a substanced formed as bloodstains age, and produces a luminescence which is best observed in the dark. The luminescence lasts for several minutes and can be photographed. Aged bloodstains tend to give more intense and longer-lasting luminescence than fresh blood, and can be re-sprayed with Luminol to be viewed again. Source: U.S. Department of Justice, Sourcebook in Forensic Serology, Immunology, and Biochemistry, Section 6.3, page 112-114.
http://www.ncids.com/forensic/serology/serology.shtml